Another study illustrated that the high levels of toxin production of TcdB were observed in complex mediums such as brain and heart infusion broth.
2.
Cultivation in tryptic soy broth ( TSB ) ], TSB with 1 % glucose, brain heart infusion broth, or Columbia broth with 2 % NaCl favors the production of CP; cultivation on Columbia salt agar plates is suboptimal.
3.
In order to purify toxin B from " C . difficile " cell cultures, brain heart infusion broth is used because it promotes the synthesis of toxin B . The filtration method facilitates purification of toxin B from the supernatant of " C . difficile ".
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